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![Phosphate Buffer, pH 7.6 [1.0 M] - 500 ml](https://m.media-amazon.com/images/I/31Hra4HFjcL.jpg)
Nº de artículo:
145284344
Phosphate Buffer, pH 7.6 [1.0 M] - 500 ml
Nº de artículo:
145284344
USD 103
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What Stands Out
Optimal pH Stability
Maintains pH 7.6 for reliable experiments, ideal for a variety of biochemical applications. Ensures consistent results, reducing variability in sensitive protocols.
Convenient Size
500 ml volume is perfect for both laboratory use and educational settings, minimizing waste while providing sufficient quantity for multiple experiments.
High Purity Formula
Crafted with high-quality reagents to ensure precision and accuracy, essential for researchers needing dependable buffer solutions for critical experiments.
Detalles de producto
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Biochemists
Ideal for experiments requiring stable pH conditions, especially in enzyme activities and protein studies.
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Microbiologists
Useful in culture media preparation where maintaining a neutral pH is critical for microbial growth.
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Laboratory Technicians
Essential for routine tests and assays requiring buffered solutions to minimize pH fluctuations during experiments.
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Acidic Solutions
Not suitable for applications requiring acidic pH adjustments, as this buffer maintains a higher pH level.
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High-temperature Experiments
May not maintain stability in extremely high temperatures, affecting buffer performance and pH accuracy.
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Non-biological Uses
Less effective in industrial applications or settings that do not involve biological samples or reactions.
DESCRIPCIÓN DEL PRODUCTO
Phosphate Buffer, pH 7.6 [1.0 M] - 500 ml
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Preguntas y respuestas de los clientes
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Pregunta:
What is Phosphate Buffer, pH 7.6 used for?
Respuesta: Phosphate Buffer, pH 7.6 is primarily used in biochemical and molecular biology experiments to maintain a stable pH environment. It's crucial for enzymatic reactions, cell culture, and various laboratory procedures where pH stability is essential. By providing a consistent pH, it ensures that biological processes occur without disruption, which is particularly important during experiments that involve sensitive biological materials. -
Pregunta:
How is Phosphate Buffer prepared?
Respuesta: To prepare a Phosphate Buffer at pH 7.6, you'll need to mix specific amounts of monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4). By adjusting their concentrations, you can achieve the desired pH. It's important to use high-purity reagents to ensure optimal performance in your experiments. This preparation can be particularly beneficial for labs conducting experiments requiring stringent pH control, ensuring that results are reproducible. -
Pregunta:
Can Phosphate Buffer be used for cell culture?
Respuesta: Yes, Phosphate Buffer, pH 7.6 is often used in cell culture applications. It helps maintain the physiological pH that cells thrive in, allowing for optimal growth and function. This buffer not only supports cellular integrity but also provides a stable environment when culturing sensitive cells, making it an essential component for researchers working in cellular biology and biotechnology. -
Pregunta:
What is the significance of the pH value in Phosphate Buffer?
Respuesta: The pH value of 7.6 in Phosphate Buffer is significant because it closely mimics physiological conditions, making it suitable for many biological experiments. The specific pH helps prevent denaturation of proteins and enzymes sensitive to pH changes. In laboratories, maintaining this pH is critical, particularly in experiments that involve biological samples needing precise conditions for optimal reactions. -
Pregunta:
What are the storage requirements for Phosphate Buffer?
Respuesta: Phosphate Buffer, pH 7.6 should be stored in a cool, dry place and ideally in a sealed container to prevent contamination. Although it is relatively stable, long-term exposure to light or varying temperatures can affect its pH and efficacy. Proper storage ensures that you can rely on its performance for consistent results in your experiments, especially for sensitive applications. -
Pregunta:
Is Phosphate Buffer suitable for molecular biology techniques?
Respuesta: Absolutely, Phosphate Buffer, pH 7.6 is frequently used in various molecular biology techniques including PCR, gel electrophoresis, and protein assays. Its ability to maintain a stable pH is crucial during these processes, as fluctuations can lead to erroneous results. Many researchers incorporate this buffer to ensure accuracy and reliability in their molecular biology experiments. -
Pregunta:
Why is a 1.0 M concentration of Phosphate Buffer significant?
Respuesta: A 1.0 M concentration of Phosphate Buffer signifies that it has a high buffering capacity, meaning it can resist changes in pH more effectively than lower concentrations. This is particularly beneficial in experiments where the addition of acids or bases might otherwise alter the pH. In practice, this allows for more precise experimental conditions, making it ideal for trials requiring strict pH regulation. -
Pregunta:
Can I use Phosphate Buffer for biochemical assays?
Respuesta: Yes, Phosphate Buffer, pH 7.6 is commonly used in various biochemical assays due to its stability and compatibility with many biological molecules. It creates an optimal environment for reactions involving enzymes and substrates, minimizing interference from pH fluctuations. Researchers often choose this buffer for enzyme activity assays to ensure results are both reliable and reproducible. -
Pregunta:
How do I determine the correct volume of Phosphate Buffer to use in experiments?
Respuesta: The correct volume of Phosphate Buffer to use will depend on the specific requirements of your experiment, including the concentration of reactants and the total volume of the reaction mixture. Typically, researchers start with a buffer volume that makes up about 10-20% of the total reaction volume. This ensures that pH is effectively stabilized, allowing for accurate measurement of results throughout the experiment. -
Pregunta:
Where can I buy Phosphate Buffer, pH 7.6 [1.0 M] - 500 ml in Puerto Rico?
Respuesta: You can buy Phosphate Buffer, pH 7.6 [1.0 M] - 500 ml on Ubuy. Ubuy offers a wide range of scientific products, making it convenient for researchers and professionals looking for high-quality lab supplies. By choosing Ubuy, you can ensure that you are getting a reliable product that meets your laboratory needs.
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USD 103
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características y beneficios
- Comprehensive range of biological buffers available.
- Manufactured in an ISO 9001 and 13485 certified facility in the USA.
- Produced under stringent Good Manufacturing Practices.
- Rigorous quality control tests confirm product integrity.
- Phosphate Buffer (pH 6.5) ideal for immunochemical staining.
- Essential for preserving living tissues during surgeries.