0 ratings
Nº de artículo:
145284746
NP40 Cell Lysis Buffer 2X, 7.4 pH, 250mL
Nº de artículo:
145284746
USD 66
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from Estados Unidos
0 ratings
Escribe una opinión
En stock
Importado de la tienda USA
QTY:
Haz tu pedido ahora y recíbelo por ahí Jueves, Septiembre 03
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Our Top Logistics Partners
Manufactured in an ISO 9001 and 13485 certified facility in the USA, ensuring the highest quality for your research needs.
Fast
Shipping
Free
Return*
Secure Packaging
100% Original Products
PCI DSS Compliance
ISO 27001 Certified
What Stands Out
Dual Concentration
The NP40 Cell Lysis Buffer is formulated at a 2X concentration, ensuring efficient and effective cell lysis in fewer steps, saving time for researchers while maintaining optimal results.
pH Stability
Maintaining a pH of 7.4, this buffer provides a controlled environment, crucial for preserving protein integrity during lysis, making it ideal for sensitive experiments and applications.
Generous Volume
With 250mL of solution, there is ample buffer for numerous applications, reducing the frequency of repurchasing and ensuring that labs can perform multiple experiments seamlessly.
Detalles de producto
| Brand | eCon-Lab-Supply |
| Item Weight | 4 ounces (113.4 grams) |
| Item Volume | 250 Milliliters |
| Package Information | Bottle |
| Item Form | Liquid |
Who Should Buy?
-
Molecular Biologists
Ideal for extracting proteins from cells to facilitate downstream molecular biology applications.
-
Biochemistry Laboratories
Useful in enzymatic studies where cell lysis is needed to release cellular components.
-
Research Institutions
Beneficial for researchers conducting studies on cellular processes and protein interactions.
-
General Consumers
Not suitable for non-laboratory individuals due to specialized usage and safety considerations.
DESCRIPCIÓN DEL PRODUCTO
NP40 Cell Lysis Buffer 2X, 7.4 pH, 250mL
¿Tienes alguna consulta? Chatea con nosotros
Preguntas y respuestas de los clientes
-
Pregunta:
What is NP40 Cell Lysis Buffer and how is it used?
Respuesta: NP40 Cell Lysis Buffer is a specialized solution designed for the efficient lysis of cells to extract cellular components such as proteins and nucleic acids. It operates at a neutral pH of 7.4 and is recommended for use in cell biology experiments where high yield and purity are critical. This buffer can disrupt both cell membranes and nuclear membranes, effectively releasing contents for further analysis. It is widely used in research laboratories for applications like Western blotting, immunoprecipitation, and enzyme assays. -
Pregunta:
What are the key components of NP40 Cell Lysis Buffer?
Respuesta: The NP40 Cell Lysis Buffer contains Nonidet P-40 (NP-40), a non-ionic detergent, along with salts and buffering agents that maintain the pH at 7.4. These components work synergistically to solubilize membrane proteins without denaturing them, which is crucial for downstream applications like SDS-PAGE or mass spectrometry. The balance of these ingredients is tailored to provide optimal lysis conditions while preserving protein functionality, making it a popular choice for life science research. -
Pregunta:
Can NP40 Cell Lysis Buffer be used for different cell types?
Respuesta: Yes, NP40 Cell Lysis Buffer is versatile and can be applied to various cell types, including mammalian, yeast, and bacterial cells. Its formulation allows for efficient lysis regardless of the cellular composition. This makes it an excellent tool in diverse experimental setups, such as studying protein interactions in mammalian cells or extracting plasmids from bacterial cells. However, it's recommended to optimize conditions based on specific cell types and experimental goals. -
Pregunta:
How should NP40 Cell Lysis Buffer be stored?
Respuesta: NP40 Cell Lysis Buffer should be stored at 4°C to maintain its stability and effectiveness. For longer storage, aliquoting the buffer into smaller tubes can help minimize repeated freeze-thaw cycles, which may compromise its integrity. Always check for any signs of precipitation or discoloration before use. Proper storage extends its viability, ensuring consistent performance in experiments over time, especially in high-throughput applications. -
Pregunta:
What precautions should be taken while using NP40 Cell Lysis Buffer?
Respuesta: When using NP40 Cell Lysis Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye contact with the buffer. Additionally, work in a well-ventilated area to avoid inhaling vapors. Ensure that all reagents are at the correct temperature prior to use and that your lab solutions are prepared under sterile conditions to prevent contamination. These precautions ensure a safe and controlled experimental environment. -
Pregunta:
How does NP40 Cell Lysis Buffer affect protein assays?
Respuesta: NP40 Cell Lysis Buffer is designed to maintain protein stability while extracting them from cells. It minimizes protein denaturation, which is crucial for assays such as Western blotting or ELISA. This buffer allows for the recovery of functional proteins, making it highly suitable for assays where protein activity is determined. Users often find that using this buffer leads to more consistent and reproducible results in their analyses. -
Pregunta:
Is NP40 Cell Lysis Buffer suitable for DNA extraction?
Respuesta: While NP40 Cell Lysis Buffer is primarily developed for protein extraction, it can also aid in DNA extraction by breaking down cell membranes that house both proteins and nucleic acids. However, to ensure purity, it's advisable to perform a phenol-chloroform extraction following lysis. This approach allows for the recovery of high-quality DNA suitable for applications such as PCR. Researchers often use this buffer to facilitate simultaneous protein and DNA study within the same sample. -
Pregunta:
Are there any alternatives to NP40 Cell Lysis Buffer?
Respuesta: Yes, there are several alternatives to NP40 Cell Lysis Buffer, including Triton X-100 and SDS-based lysis buffers. Each of these alternatives may have specific advantages depending on the application. For instance, Triton X-100 is milder and less likely to disrupt protein structure, making it suitable for applications requiring intact proteins. In contrast, SDS is more effective for complete denaturation of proteins. Researchers often choose a buffer based on their specific experimental needs and the type of analysis they plan to perform. -
Pregunta:
How much NP40 Cell Lysis Buffer should I use per sample?
Respuesta: The optimal volume of NP40 Cell Lysis Buffer typically depends on the type and number of cells being lysed. A general recommendation is to use 100-300 µL of the buffer per 1-10 million cells. Adjusting the volume may be necessary based on cell density and the specific requirements of your assay. Researchers find that using the right amount ensures efficient lysis while preventing the dilution of key cellular components, leading to more reliable experimental outcomes. -
Pregunta:
Where can I buy NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL in Puerto Rico?
Respuesta: You can purchase NP40 Cell Lysis Buffer (2X), 7.4 pH, 250mL from Ubuy in Puerto Rico. Ubuy offers a reliable platform for sourcing laboratory reagents and supplies, making it a convenient choice for researchers and scientists needing high-quality buffers and reagents. Ensure you check their website for availability and details on your specific needs.
eConLabSupply Buffers Editorial Review
No se encontraron reseñas editoriales
Customer Reviews & Ratings
1 valoraciones de los clientes
-
5 estrella
0%
-
4 estrella
100%
-
3 estrella
0%
-
2 estrella
0%
-
1 estrella
0%
Revisar este producto
Comparte tus ideas con otros clientes
Platform Trust & Buyer Confidence
MA
Mohammad
Verified buyer
“Excellent quality and original too,when ubuy send original things I will appreciate that ,I very satisfied thank you”
23 July 2026 · via Trustpilot
CK
Chitra
Verified buyer
“The order and delivery progress was communicated very well. Package arrived within the estimated time. Products arrived as expected in good condition.”
22-Jul-26 · via Trustpilot
K
kund
Verified buyer
“Good supply of products. Safe payment methods, and shipment worldwide! Genuine products.”
23-Jul-26 · via Trustpilot
OO
Onesi
Verified buyer
“Was my first time buying a product from Ubuy, but I found it so helpful. This is reliable. Gonna place a new order!”
22 July 2026 · via Trustpilot
SJ
SAO JOHN
Verified buyer
“Ubuy is a great online platform to buy stuff. Reliable, fast delivery time and great value for money. I've been using Ubuy online shopping platform for three years now and will continue to do so.”
23 July 2026 · via Trustpilot
Pago seguro
Global Delivery
Easy Returns
Genuine Products
Product Price History
Información importante
- Limitaciones: Para los productos enviados al extranjero, ten en cuenta que cualquier garantía del fabricante puede no ser válida; las opciones de servicio del fabricante pueden no estar disponibles; los manuales del producto, las instrucciones y las advertencias de seguridad pueden no estar en los idiomas del país de destino; los productos (y los materiales que los acompañan) pueden no estar diseñados de acuerdo con las normas, especificaciones y requisitos de etiquetado del país de destino; y los productos pueden no ajustarse al voltaje del país de destino y a otras normas eléctricas (lo que requiere el uso de un adaptador o convertidor, si procede). El destinatario es responsable de asegurarse de que el producto puede ser importado legalmente al país de destino. Cuando hagas un pedido a Ubuy o a sus filiales, el destinatario es el importador registrado y debe cumplir todas las leyes y normativas del país de destino.
- No todos los productos que aparecen en Ubuy están a la venta, ya que Ubuy es un motor de búsqueda a nivel mundial. Los productos están sujetos a las normas de exportación/comercio.
USD 66
Haz tu pedido ahora y recíbelo por ahí Jueves, Septiembre 03
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Secured transaction
PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.
Our Top Logistics Partners
Fastest cross-border delivery
características y beneficios
- Comprehensive range of biological buffers.
- Ideal for research, diagnostics, drug development, and manufacturing.
- Certified facility guarantees top-tier quality.
- Designed for protein research and molecular biology.
- NP40 Cell Lysis Buffer is suitable for key assays like ELISA and Western blotting.
- Strict adherence to Good Manufacturing Practices ensures reliability.
Ubuy Assurance
Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns *, and secure packaging on every order.